Fufang Lishao Pills Improves Chronic Migraine by Inhibiting Neuroinflammation through the Blockage of JAK2/STAT3 Pathway in Cortex of Rat with Nitroglycerin Induction
10.11842/wst.2018.12.021
- VernacularTitle:复方藜芍片通过阻断JAK2/STAT3通路抑制皮层神经炎症改善硝酸甘油致大鼠慢性偏头痛
- Author:
Jianmei YANG
1
;
Haiyan DENG
;
Kun LI
;
Xingyu WANG
;
Lei CHEN
;
Jiawei LI
;
Yun RU
;
Zhengpin GU
;
Ying XU
Author Information
1. 上海市徐汇区中心医院 上海 200031
- Keywords:
Migraine;
Fufang Lishao Pills;
neuroinflammation;
JAK2/STAT3 pathway
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2018;20(12):2224-2230
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effects of Fufang Lishao Pills (FFLSP) on the proinflammatory factors, pain-relatedproteins and JAK2/STAT3 signaling pathways in nitroglycerin-induced chronic migraine model, and exploring thepharmacological target and mechanism of FFLSP on chronic migraine rats. Methods: Male Sprague-Dawley rats wererandomly divided into 6 groups: Control, Migraine, FFLSP-L (420 mg·kg-1), FFLSP-M (840 mg·kg-1), FFLSP-H (1680mg·kg-1) and Flunarizine Hydrochloride group (FH, 1 mg·kg-1) . Chronic migraine model was made by subcutaneousinjection of nitroglycerin (10 mg·kg-1) once every 3 days for 5 times. The rats were treated with FFLSP by intragastricadministration once a day for 30 days. Western blot was used to detect the protein expression of iNOS, COX-2, CGRPand c-Fos and the phosphorylation levels of JAK2 and STAT3 in cortex of rats. The production of IL-1β and TNF-αwere detected by enzyme linked immunosorbent assay (ELISA) . Results: Compared with Control rats, and the level ofiNOS, COX-2, CGRP, c-Fos expression (P ＜ 0.01) and IL-1β, TNF-α production remarkably up-regulated (P ＜ 0.05), and the phosphorylation of JAK2 and STAT3 also significantly increased in cotex of Migrainerats (P ＜ 0.01) . However, compared with Migrainerats, the levels of iNOS, COX-2, CGRP, c-Fos expression and IL-1β, TNF-α productionobviously declined (P ＜ 0.05; P ＜ 0.01), and the phosphorylation level of JAK2 and STAT3 showed a significantlydecrease in the cortex of Migraine rats with FFLSP treatment (P ＜ 0.01) . Conclusion: This study demonstrates that thepharmacological mechanism of FFLSP in improving chronic migraine may be achieved by inhibiting neuroinflammationthrough the blockage of JAK2/STAT3 pathwayin the cortex of rat with nitroglycerin induction.
