Selection and Identification of Producing Alkaloid Endophytic Fungi From Fritillaria anhuiensis
- VernacularTitle:皖贝母产生物碱内生真菌的筛选与鉴定
- Author:
Yong XU
1
;
Chenwei DAI
;
Chaoyang DONG
;
Biao CAI
Author Information
- Keywords: Fritillaria anhuiensis; endophytic fungi; alkaloid
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2018;20(11):2021-2026
- CountryChina
- Language:Chinese
- Abstract: Objective: To select alkaloid-producing endophytic fungi strains from Fritillaria anhuiensis. Methods: The endophytic fungi were selected from the bulbus of Fritillaria anhuiensis, by tissue explants, thin layer chromatography, the high performance liquid chromatography and high performance liquid chromatography-evaporative light scattering detection, morphologic observation and molecular biological methods. Results: One strain as an endophytic fungi capable of producing alkaloid was selected from the bulbus of Fritillaria anhuiensis, and was classified as Alternaria tenuissima. Conclusion: The selected alkaloid-producing endophytic fungi strain bm-2 from Fritillaria anhuiensis provide theoretical foundation for solving the problem of the deficient medical resources of Fritillaria anhuiensis.
