Study on the Effects and Mechanism of Yinlan Tiaozhi Formula on Macrophage Foaming
- VernacularTitle:银蓝调脂胶囊对巨噬细胞泡沫化的抑制作用及机制研究
- Author:
Dane HUANG
1
;
Ruyue LI
;
Dake CAI
;
Nan YAO
;
Haining GAN
;
Xiaohui ZENG
;
Yuxing CHEN
Author Information
- Keywords: Yinlan Tianzhi formula; foam cell; cholesterol efflux; inflammation
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2018;20(11):2014-2020
- CountryChina
- Language:Chinese
- Abstract: Objective: To investigate the inhibitory effect of macrophage foaming by Yinlan Tianzhi formula (YLTZ) and to explain its effects on lipid-induced inflammation and LXRα-ABCA1 signal pathway. Methods: The model of macrophage foaming was induced by incubating the RAW264.7 cells or BMMs with ox-LDL (50 mg·L-1). The serum containing YLTZ was prepared. The cells were divided into blank group, model group, and drug group. After drug intervention, MTT method was used to detect cell proliferation. The lipid accumulation in cells was observed by oil red O staining, and GPO-PAP method was used to determine the total cholesterol content in cells. Protein and mRNA levels were determined by Western blot and RT- qPCR. Results: Compared with control group, after YLTZ treatment, the lipid level was significantly decreased, and the level of mRNA and protein of LXRα and ABCA1 were significant increased. The expression of inflammatory factor COX2 and iNOS was significantly decreased. Conclusion: YLTZ inhibits macrophage foaming through enhancing LXRα-ABCA1 pathway and suppressing of inflammatory response.
