Content Determination and Cluster Analysis of 3 Lignans from Cortex Magnoliae Officinalis in Different Regions
- VernacularTitle:不同产地厚朴药材中3种木脂素类成分含量测定及聚类分析
- Author:
Wenguang JING
1
,
2
;
Quan ZHANG
;
Jie DU
;
Jiyong WANG
;
Xiaobo SUN
;
Qingshan LAN
Author Information
- Keywords: UPLC; Cortex Magnoliae Officinalis; simultaneous determination; lignans
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2018;20(10):1822-1827
- CountryChina
- Language:Chinese
- Abstract: Objective: To establish the method of simultaneous determination of contents of honokiol, magnolol and piperitylmagnolol in Cortex Magnoliae Officinalis from different regions, which could provide evidence for the quality control evaluation of Cortex Magnoliae Officinalis. Methods: The quantitative analysis was performed with a column of Waters Acquity UPLC BEH-Cl8 (2.1 mm×50 mm, 1.7 μm) by UPLC-PDA, and eluted with a mobile phase of 0.2％formic acid solution (A)-methanol (B) in a gradient mode (0-2 min, 65％-65％ B; 2-3 min, 65％-75％ B; 3-5 min, 75％-84％ B; 5-9 min, 84％-90％ B) under a flow rate of 0.5 mL·min-1. The column temperature was set at 35℃, and the detection wavelength was 294 nm and the injection volume was 1 μL. Results: The 3 lignan components were completely separated and could be separated well from other components. The honokiol, magnolol and piperitylmagnolol showed a good linear relationship with chromatographic peak area in range of 20.3-406.0, 15.2-304.0, 5.6-112.0 ng, respectively.The average recoveries were 91.75％, 93.86％, 95.15％ with the RSDs were 1.75％, 1.88％, 1.91％ (n = 9), respectively.Conclusion: Contents of the 3 constituents from different place were significantly different and this method is simple and effective, which can be used to quality control of Cortex Magnoliae Officinalis.
