Study on HPLC Fingerprint of Terminalia chebular Retz Based on Chemometrics Analysis
10.11842/wst.2018.09.023
- VernacularTitle:基于化学计量学分析的诃子药材高效液相色谱指纹图谱研究
- Author:
Wenyi LIANG
1
;
Qi QI
;
Linjin LIANG
;
Ting YE
;
Kun ZHOU
;
Ping JIAN
;
Lanzhen ZHANG
Author Information
1. 北京中医药大学中药学院 北京 102488
- Keywords:
Terminalia chebular Retz;
fingerprints;
chemometrics;
partial least squares discriminant analysis
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2018;20(9):1645-1649
- CountryChina
- Language:Chinese
-
Abstract:
This paper was aimed to establish the HPLC fingerprint of Terminalia chebular Retz The analysis was performed on Agilent ZORBAX SB-Aq C18 column (4.6×250 mm, 5 μm) . The mobile phase was 0.1％ phosphate watermethanol solution with gradient elution. The flow rate was 1 mL·min-1. The temperature was maintained at 35℃. The detection wavelength was 270 nm. The injection volume was 20 μL. The chromatographic data of 10 samples was analyzed by similarity of chromatographic data, SPSS software and SIMCA software. The results showed that there were17 common peaks in the diagram. The similarity of 10 samples was between 0.984 and 0.999, which showed that there was no significant difference among these chemical components. The samples were classified into four types according to cluster analysis, which shared the similar results as the principal components analysis (PCA) and partial least sq uares analysis (PLS-DA) . VIP analysis result showed that peak 5 (methyl gallate), 11 and 1 (gallic acid), 10, 12 may be the main chromatographic peaks to identify the samples. It was concluded that chemometrics analysis could be used to analyze, find and distinguish the marker ingredients of Terminalia chebular Retz. This method was accurate and stable, which can provide a reference for the quality control and evaluation of Terminalia chebular Retz.
