Study on Rapid Identification of Constituents of Tibetan Medicine Terminalia chebular Retz. by HPLC/LTQ-Orbitrap MSn
10.11842/wst.2018.09.021
- VernacularTitle:藏药诃子化学成分的高效液相色谱-质谱联用技术快速鉴定研究
- Author:
Ping JIAN
1
;
Qi QI
;
Kun ZHOU
;
Wenyi LIANG
;
Linjin LIANG
;
Ting YE
;
Lanzhen ZHANG
Author Information
1. 北京中医药大学中药学院 北京 102488
- Keywords:
Terminalia chebular Retz;
LTQ-Orbitrap high-resolution mass spectrometers;
phenolic acids;
hydrolysable tannins
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2018;20(9):1627-1637
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this work was to identify and analyze the chemical constituents of Terminalia chebular Retz by establishing high performance liquid chromatography-linear ion trap/electrostatic field orbitrap combined high resolution mass spectrometry (HPLC-LTQ-Orbitrap MS) technique. With the application of C18 reverse phase column (4.6 mm×250 mm, 5 μm), gradient elution was performed with methanol and water (0.2％ acetic acid) as mobile phase.In negative ionization mode, the data of LTQ-Orbitrap was collected. Accurate molecular mass of molecular ion peaks and fragment ions provided by the high-resolution mass spectrometry were compared with the literature and the reference substance to determine the possible structure of the compound. The results indicated that 62 compounds from the extract of Terminalia chebular Retz. were identified, including acids and hydrolysable tannins (60), triterpenoids (2) . There were14 compounds were detected from this specie for the first time. It is proved as an effective method to provide reference for Chemical composition mass spectrometry and the quality control in further phytochemical studies of Terminalia chebular Retz.
