Comprehensive Evaluation of the Quality of Tibetan Medicine Triphala's Tannin Parts Based on There Characteristic Components and the Total Tannin Content
10.11842/wst.2018.09.019
- VernacularTitle:基于多成分和总鞣质测定的藏药大三果及其鞣质部位质量研究
- Author:
Ting YE
1
;
Yaping CUI
;
Linjin LIANG
;
Wenyi LIANG
;
Ping JIAN
;
Kun ZHOU
;
LIngfang WU
;
Shi LI
;
Qi QI
;
Lanzhen ZHANG
Author Information
1. 北京中医药大学中药学院 北京 102488
- Keywords:
Triphala;
Tannin fraction;
spectrophotometry;
high performance liquid chromatography;
content determination
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2018;20(9):1615-1621
- CountryChina
- Language:Chinese
-
Abstract:
To determine there characteristic components content of Tibetan Medicine Triphala through establishing a HPLC method and its total tannin content through spectrophotometry. The chromatographic column of Agilent ZORBAX SB-Aq (250 × 4.6 mm, 5 μm) with methanol-0.1％ formic acid/water as the mobile phase, the flow rate 1 mL/min, and the detection wavelength 270 nm was applied to determine the Gallic acid, colijing and ellagic acid content in medicinal materials and the tannin parts of Tibetan Medicine Triphala. With the gallic acid as control group, total tannin content of Triphala and its tannin parts was determined through spectrophotometry. It revealed in the HPLC test that the linear range of gallic acid, coracine and ellagic acid was 0.91-4.55 μg, 0.274-1.368 μg and 0.329-2.634 μg respectively. It also showed that the average recovery rates of the three components in the medicinal materials were 101.06％, 101.72％and 100.27％ respectively. And the average recovery rates of the three components in the tannins were 100.4％, 100.85％and 101.70％ respectively. The result of spectrophotometry showed that gallic acid was linear in1.008-10.08 μg·mL-1, and that the recovery rate of medicinal materials and tannin parts were 100.25％ and 100.52％ respectively. The method is rapid, accurate and repeatable, and it can provide basis for the quality control of Tribescens and its tannins.
