Protective Mechanism of Preventative Moxibustion at Tianshu Acupoint (ST25) on Intestinal Barrier in UC Rats
- VernacularTitle:艾灸预处理"天枢"穴对溃疡性结肠炎大鼠肠黏膜屏障保护的机制研究
- Author:
Zhe MA
1
,
2
;
Zhenzhen FANG
;
Huangan WU
;
Muen GU
;
Ling YANG
;
Qin QI
;
Di WANG
;
Kunshan LI
;
Huirong LIU
;
Yan HUANG
;
Yuan LU
Author Information
- Keywords: Preventative moxibustion; ulcerative colitis; intestinal mucosal barrier; inflammatory reaction
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2018;20(9):1555-1563
- CountryChina
- Language:Chinese
- Abstract: Objective: To observe the effect of anti-inflammatory by preventative moxibustion at Tianshu Acupoint (ST25) on UC rats, and to investigate the protective mechanism of preventative moxibustion at Tianshu Acupoint (ST25) onintestinal mucosal barrier in UC rats. Methods: A total of 28 male Sprague-Dawley rats were randomly divided into thenormal group (NC), the ulcerative colitis group (UC), the herb-partitioned moxibustion pretreatment group (HM+P), thewarming moxibustion pretreatment group (WM+P), with 7 in each group. The HM+P and the WM+P were treated withherb-partitioned moxibustion and warming moxibustion for 7 days before the model was established. After pretreatment, the UC, the HM+P and the WM+P were given 4％ dextran sodium sulfate solution for 7 days. Observe the rat pathologicalchanges of colon tissue by HE staining, detect the protein expression in rat colon tissue by immunohistochemistry andwestern blot. Results: compared with the NC group, the pathological scores were increased, the expression of occludin, JAM1, MUC2, ZO-1 were decreased and the expression of NF-κBp65, IL-1βincreased in UC group (P ＜ 0.05);compared with the UC group, the pathological score decreased, the protein expression of occludin, JAM1, MUC2, ZO-1 increased, the protein expression of NF-κBp65, IL-1βdecreased in HM + P group (P ＜ 0.05), the Pathological scoredecreased, the Protein expression of occludin, MUC2, ZO-1 increased, the Protein expression of IL-1β decreased inWM + P group (P ＜ 0.05) . Conclusion: The herb-partitioned moxibustion pretreatment and the warming moxibustionpretreatment could increase the expression of intestinal barrier related proteins in UC rats, it may be one of themechanisms of moxibustion to relieve colonic inflammation of UC.
