Study on Diversity of Intestinal Flora of Patients with Large Intestine Damp Heat Syndrome and Ulcerative Colitis By High-Throughput Sequencing
- VernacularTitle:基于高通量测序技术的溃疡性结肠炎大肠湿热证患者肠道菌群多样性的研究
- Author:
Panghua DING
1
,
2
;
Juniang LI
;
Yi GUO
;
Tangyou MAO
;
Xingjie ZHAO
Author Information
- Keywords: Intestinal flora; ulcerative colitis; large intestine damp heat syndrome; high throughput sequencing
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2018;20(6):967-973
- CountryChina
- Language:Chinese
- Abstract: Objective: To explore the difference of intestinal flora between groups of ulcerative colitis and large intestine dampness heat syndrome and healthy group. Methods: A total of 14 stool samples from patients with ulcerative colitis and large intestine dyspepsia syndrome and 14 samples of healthy people were collected. Amplification was designed based on the 16 S r DNA V3-V4 region of the bacteria. Illumina HiSeq 2500 platform was used for high-throughput sequencing.The ultimately sample species information was get by Reads splicing, OTUs (operational taxonomic units) clustering, species annotation, the analysis of alpha diversity and the analysis of principal component. Results: There was a significant difference in intestinal flora between the ulcerative colitis group and the healthy group (P=0.008 ＜ 0.01); and the diversity of intestinal flora in patients with ulcerative colitis and large intestine dyspepsia syndrome was higher than that of healthy people. There a significant difference in the richness degree of intestinal flora patients with ulcerative colitis and large intestine dampness syndrome and the healthy group. Intestinal flora centered on Lactobacillus、Lactobacillaceae、Erysipelotrichaceae、Erysipelotrichales and Akkermansia bacteria was rich in patients with ulcerative colitis. Conclusion: There are significant differences in intestinal flora diversity and bacterial structure between patients with ulcerative colitis and large intestine dampness syndrome and the healthy group.
