Simultaneous Determination of Quercetin and Mignonette in Gnaphalium affine by HPLC
10.11842/wst.2018.04.013
- VernacularTitle:高效液相色谱法同时测定鼠曲草中槲皮素与木犀草素的含量
- Author:
Zanshan XU
1
;
Xiaohua LIN
Author Information
1. 厦门医学院药学系 厦门 361023
- Keywords:
Gnaphalium affine;
Quercetin;
Mignonette;
HPLC
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2018;20(4):553-557
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the simultaneous determination of quercetin and mignonette in Gnaphalium affine. Methods The determination was performed on Diamonsil C18 (2) column (4.6 mm×250 mm, 5 μm), mobile phase was methanol-0.4％ phosphoric acid solution (42:58), detection wavelength was set at 360 nm, column temperature was 30℃, flow rate was 1 mL · min-1 and the injection volume was 20 μL. Results The linear rages of quercetin and mignonette were 3.588-35.880 μg · mL-1 (R2=0.999 6) and 1.294-12.940 μg · mL-1 (R2=0.999 4) respectively. Their average recoveries (n=6) were 99.51％ (RSD=0.31％) and 99.74％ (RSD=2.28％) respectively.Quercetin Content was 0.39-0.58 mg·g-1, mignonette content was 0.17-0.27 mg·g-1.Conclusion The method is simple, accurate, reliable and repeatable, thus it can be used for the quality control of Gnaphalium affine.
