Research on Reversal Effects in Cisplatin-resistant Human Lung Adenocarcinoma Cell Line A549/DDP by Standard Substances from Traditional Chinese Medicine
- VernacularTitle:中药标准品对人肺癌耐药细胞A549/DDP的逆转效果研究
- Author:
Xinjie YANG
1
,
2
;
Jingxiao WANG
;
Lin WANG
;
Xiaoyi CHI
;
Jiaqi CHI
;
Tian ZHOU
;
Tonghua LIU
;
Kaiwen HU
Author Information
- Keywords: Standard substances; tariquidar; A549 Cell Line; A549/DDP Cell Line; chemoresistance
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2018;20(4):521-526
- CountryChina
- Language:Chinese
- Abstract: Objective: To investigate the reversal effects in A549/DDP by standard substances (SS) from Traditional Chinese Medicine. Methods: Cell proliferation assays were performed to investigate the Resistant Index of A549/DDP and its tolerance to selected SS. The working concentrations of SS, IC5 calculated by nonlinear regressions, were applied as reversal doses to investigate the effects. Results: The resistant index of A549/DDP was 31.79. Tetramethylpyrazine and Matrine were well tolerated. Berberine hydrochloride, Dauricine, Oridomin exhibited dose-depend inhibitory effects on A549/DDP cell line. The working concentrations of them could effectively reverse the resistance of A549/DDP cell line to DDP. (P＜0.01) . Conclusion: The selected standard substances from Traditional Chinese Medicine were capable to reverse the resistance of A549/DDP cell line to DDP.
