MicroRNA inhibits VEGF expression in ovarian cancer cells and its effect on tumor cell proliferation
10.3760/cma.j.issn.1674-6090.2019.03.013
- VernacularTitle:microRNA抑制卵巢癌细胞VEGF表达及其对肿瘤细胞增殖的影响
- Author:
Zhongna YU
1
;
Yaner DONG
Author Information
1. 宁波大学附属鄞州医院妇产科 315040
- Keywords:
microRNA;
Ovarian cancer;
Cell VEGF;
Tumor cell proliferation
- From:
Chinese Journal of Endocrine Surgery
2019;13(3):233-236
- CountryChina
- Language:Chinese
-
Abstract:
Objective TostudytheeffectofmicroRNA(miRNA) on the expression ofVEGF in ovarian cancer cells and its effect on tumor cell proliferation,in order to provide a new way for clinical diagnosis and treatment.Methods The two transfected cells were divided into HO-8910-H1-VEGF group and HO-8910-H1 group,and untransfected cells were referred to as the control group(HO-8910 group) by MTr(tetrazolium salt) method.The effect of miRNA on the proliferation of ovarian cancer cells was observed by periodic analysis.Results The expression of VEGF in HO-8910 cells was significantly decreased after transfection with specific small hairpin miRNA plasmid expression vector,ie,the expression of VEGF in HO-8910 H1-VEGF group was decreased compared with HO-8910 H1 group and HO-8910 group;Indirect immunofluorescence assay showed that the HO-8910-H1-VEGF group showed weak fluorescence intensity,but the HO-8910 H1 group and HO-8910 group had stronger fluorescence intensity.Cells in the HO-8910-H1-VEGF group that were blocked in the phase G1 (inter val phase 1) increased by 24％,and cells in phase S (DNA synthesis phase) showed a 21％ reduction.Conclusion MicroRNA can significantly inhibit the expression of VEGF in ovarian cancer HO-8910 cells,thereby blocking the proliferation of ovarian cancer cells and providing a theoretical basis for clinical gene therapy.
