Expression and clinical significance of FGF8b in ovarian cancer tissues
10.3760/cma.j.issn.1674-6090.2019.03.012
- VernacularTitle:FGF8b在卵巢癌组织中的表达及其临床意义
- Author:
Tao SUN
1
;
Pengli HAN
;
Fengmei LI
Author Information
1. 郑州大学附属郑州中心医院药学部
- Keywords:
FGF8b;
Ovarian cancer;
RT-PCR;
Western blot
- From:
Chinese Journal of Endocrine Surgery
2019;13(3):229-232
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of FGF8b in ovarian cancer tissue and its relationship with clinical characteristics of patients.Methods Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the FGF8b mRNA level in 28 cases of human ovarian cancer tissues and the adjacent normal tissues.Western blot method was used to detect the FGF8b protein.The relationship between the clinical characteristics of the patients was analyzed.Results The expression of FGF8b mRNA in ovarian cancer tissue (0.23±0.08) was higher than that of paraplastic tissue (0.71±0.11)(P＜0.05),and FGF8b protein expression in cancerous tissue (0.27±0.03) was higher than that in cancerous tissue (0.44±0.03)(P＜0.05).In ovarian cancer tissues,FGF8b positive rate (76.9 ％) in patients with stage Ⅲ to Ⅳ was higher than in patients with stage Ⅰ to Ⅱ (33.3％) (P＜0.05) The positive rate of lymph node metastasis (60.0％) was higher than that of non-lymphatic metastasis (27.8％)(P＜0.05).Conclusion FGF8b is significantly highly expressed in ovarian cancer tissues,which is correlated with the clinical characteristics of the ovarian cancer patients.
