Effect of gastric bypass surgery on intestinal flora and some serum indexes in rats with type 2 diabetes mellitus
10.3760/cma.j.issn.1674-6090.2019.03.002
- VernacularTitle:胃转流术对2型糖尿病大鼠肠道菌群及部分血清指标的影响
- Author:
Nan CHENG
1
;
Ju WU
Author Information
1. 大连大学附属中山医院肝胆胰腹腔镜外科 116001
- Keywords:
Gastric bypass surgery;
Type 2 diabetes mellitus;
Insulin resistance;
Intestinal flora
- From:
Chinese Journal of Endocrine Surgery
2019;13(3):181-185
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of gastric bypass (GBS) on intestinal flora and some serum indexes in spontaneous type 2 diabetic rats (GK rats),and to explore the mechanism of improving insulin resistance.Methods 30 Goto-Kakizaki rats,aged 8 weeks,were randomly divided into GBS operation group,sham operation group and diet matching group (n=10).Another 10 8-week-old male SD rats served as blank control group (free eating and drinking).The levels of fasting blood glucose(FPG) and fasting insulin(FINS) were measured and compared before and 4 weeks after operation.The insulin resistance index(HOMA-IR) was calculated before and 4 weeks after operation.Fresh feces of rats were collected 4 weeks after operation and DNA was extracted.The intestinal flora composition of each group was detected by real-time polymerase chain reaction (RT-PCR) at 4 weeks after operation.Results Four weeks after operation,FPG level in GBS group decreased from (13.83±0.35) mmol/L to (5.73±0.21) mmol/L(P＜0.05),HOMA-IR in GBS group decreased from(5.71±0.27) to (2.15±0.17) (P＜0.05).The DNA content of Firmicutes(22.71±12.81)(P=0.020),Enterobacterium spp.(3.11±4.17) (P=0.043) decreased significantly,and the DNA content of Bacteroides (30.74± 17.33) (P=0.013)increased significantly.Conclusion GBS can down-regulate the intestinal thick-walled bacterium,Enterobacteriaceae and upregulate the content of mimetic bacterium in type 2 diabetic rats,improve insulin resistance and insulin sensitivity.
