N-Acetyl-cysteine protects A549 cells in paraquat-induced epithelial-mesenchymal transition
10.3760/cma.j.issn.1671-0282.2019.06.011
- VernacularTitle:N-乙酰半胱氨酸在百草枯诱导A549细胞转分化过程中的保护作用
- Author:
Yang SONG
1
;
Hao SUN
;
Guoqin YU
;
Xiaomin ZHANG
;
Yuxuan WU
;
Jun WANG
;
Jinsong ZHANG
Author Information
1. 南京医科大学第一附属医院急诊医学科 210029
- Keywords:
Paraquat;
N-Acetyl-cysteine;
Transforming growth factor-β1;
Epithelial-mesenchymal transition
- From:
Chinese Journal of Emergency Medicine
2019;28(6):712-716
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulatory mechanism of Paraquat (PQ)-induced epithelial-mesenchymal transition (EMT) and the protective mechanism of N-acetyl-cysteine (NAC) in PQ-induced A549 cells by EMT markers (E-cadherin and α-smooth muscle actin).Methods EMT model was established by 30 μmol/L PQ-exposed A549 cells for 4 d.The application of 10 mmol/L NAC was incubated with A549 cells for 2 h in advance.Morphological changes of A549 cells were observed under light microscope.Western blot was used to detect the expression levels of TGF-β1,E-cadherin and α-smooth muscle actin.Results In PQ-induced EMT model,the PQ group showed typical morphological changes,and the cells changed from cobblestone-like epithelial appearance to spindle-shaped mesenchymal-like appearance compared to the control group.Westem blotting showed that the protein levels of TGF-β1 and α-smooth muscle actin in the PQ group were significantly increased and the protein levels of E-cadherin were decreased in the PQ group (all P＜0.05).NAC preincubation can effectively reverse the above changes caused by PQ exposure:the protein levels of E-cadherin and α-smooth muscle actin were significantly increased and decreased,respectively and morphological changes showed more cobblestonelike epithelial appearance.Conclusion Cell experiments showed that PQ exposure can cause EMT in epithelial cells,and NAC has a protective effect in this process.Thus,it may provide new ideas and strategies for the treatment of pulmonary fibrosis caused by PQ poisoning in clinical practice.
