Determination of ginsenoside Rg1 and Re in Tongru granules by HPLC
10.3760/cma.j.issn.1673-4246.2019.05.014
- VernacularTitle:HPLC法检测通乳颗粒中人参皂苷Rg1与Re含量
- Author:
Yan LI
1
;
Hongjie CHENG
;
Keming LI
Author Information
1. 山东省新泰市中医医院药学部
- Keywords:
Ginsenoside Rg1;
Ginsenoside Re;
High performance liquid chromatography;
Tongru granules;
Assay (TCD);
Quality control
- From:
International Journal of Traditional Chinese Medicine
2019;41(5):497-501
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid and efficient method for the simultaneous determination of ginsenoside Rg1 and ginsenoside Re in Tongru granules by HPLC.Methods The HPLC analysis was carried out with Agilent ZORBAX SB-C18 and acetonitrile-0.1％ phosphoric acid(15:85,V/V) as mobile phase,the flow rate was 1.0 ml/min,the column temperature was 30 ℃,injection volume was 10 μl,and the detection wavelength was 203 nm.Results The Rg1 solution of ginsenoside showed a good linear relation between 0.136-2.040 μg (r2=0.999 9).The ginsenoside Re solution presented a good linear relationship (r2=0.999 8)between 0.066-0.990 μg,with the average addition recovery of 99.3％ and 99.1％,and the relative standard deviation of 0.84％ and 0.75％(n=6),respectively.Conclusions The method is highly specific,sensitive,reproducible,simple and efficient which can be used for quality control of Tongru Granules.
