Regulation of Wnt signaling pathway on calcium hydroxide-promoted osteogenic differentiation of hDPSCs
10.3760/cma.j.issn.1673-4181.2019.02.005
- VernacularTitle:Wnt信号通路对氢氧化钙促进人牙髓干细胞成骨向分化的调控
- Author:
Luoping CHEN
1
;
Lisha ZHENG
;
Yubo FAN
Author Information
1. 北京航空航天大学生物与医学工程学院
- Keywords:
Calcium hydroxide;
Osteogenic differentiation;
Human dental pulp stem;
Wnt pathway
- From:
International Journal of Biomedical Engineering
2019;42(2):120-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of Wnt/β-catenin signaling pathway in the osteogenic differentiation of human dental pulp stem cells (hDPSCs). Methods After 14 days of the calcium hydroxide training, the cytoskeletal changes of hDPSCs, the expression of β-catenin, i.e. the key promoter of in the Wnt signaling pathway, and the cell localization were detected by laser scanning confocal technique. The Wnt signaling pathways were up-regulated and inhibited, and the osteogenic differentiation and mineralization of hDPSCs were detected by Western Blot and alizarin red staining after 14 days of the training. Results The cytoskeleton of hDPSCs was rearranged by the effect of calcium hydroxide, and theβ-catenin migration from nucleus to cytoplasm were observed. The number of calcium nodules in hDPSCs was decreased after blocking Wnt signaling pathway by Dickkopf-related protein 1 (DKK-1). The calcium hydroxide treatment can promoted dentin sialophosphoprotein (DSPP) expression in hDPSCs. Conclusions Calcium hydroxide can down-regulate the expression of canonical Wnt signaling pathway and promote osteogenic differentiation and mineralization and odontogenetic differentiation of hDPSCs.
