Synthesis and biosafety evaluation of four acryl acetyl glucosamine with cell membrane binding ability
10.3760/cma.j.issn.1673-4181.2019.02.004
- VernacularTitle:有细胞膜结合能力的四丙烯酰基乙酰氨基葡萄糖的合成与生物安全性评价
- Author:
Xiaohui WANG
1
;
Ping GAO
;
Lin CHENG
;
Bin CHEN
;
Weiwei LIU
;
Zibo YANG
;
Mengrou SHI
;
Dongdong LI
Author Information
1. 天津市医药科学研究所 300020
- Keywords:
Cell membrane;
Four acryl acetyl glucosamine;
Self agglutination gel;
Biosafety
- From:
International Journal of Biomedical Engineering
2019;42(2):115-119,138
- CountryChina
- Language:Chinese
-
Abstract:
Objective To synthesize and characterize four acryl acetyl glucosamine (DA-NAG), and to determine its biocompatibility and cell membrane binding properties, so as to provide basis for its application in medical self agglutination gels. Methods DA-NAG was synthesized by esterification reaction. The products were characterized by mass spectrometry and hydrogen spectrum. Cytotoxicity test and subcutaneous implantation test were performed on the synthesized DA-NAG. The binding properties of DA-NAG to mouse fibroblast L929 cell membrane were detected using high performance liquid chromatography (HPLC). Results The characterization of mass spectrum and hydrogen spectrum are consistent with the characteristics of DA-NAG. The product has no cytotoxicity, and the subcutaneous implantation shows that the DA-NAG can be degraded at 4 weeks without obvious stimulation to the surrounding tissues. The result of HPLC shows the binding effect between the DA-NAG and cell membrane. Conclusions DA-NAG is successfully synthesized, and it has good cytocompatibility and binding ability to cell membrane.
