Local brain hypothermia and nicotinamide phosphoribosyltransferase synergistically protect cerebral ischemia-reperfusion injury in rats
10.3760/cma.j.issn.1673-4165.2019.04.005
- VernacularTitle:局部脑低温与烟酰胺磷酸核糖基转移酶协同保护大鼠脑缺血再灌注损伤
- Author:
Xiaoduo HE
1
;
Yunxia DUAN
;
Jingfei SHI
;
Di WU
;
Yuchuan DING
Author Information
1. 首都医科大学宣武医院中美神经科学研究所
- Keywords:
Brain ischemia;
Reperfusion injury;
Nicotinamide phosphoribosyltransferase;
Hypothermia,induced;
Neuroprotection;
Disease models,animal;
Rats
- From:
International Journal of Cerebrovascular Diseases
2019;27(4):267-272
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the neuroprotective effect and its mechanism of local hypothermia combined with nicotinamide phosphoribosyltransferase (NAMPT) on cerebral ischemia-reperfusion injury in rats.Methods Fifty-four Sprague-Dawley rats were randomly divided into sham operation group,model group,NAMPT group,local hypothermia group,and combined treatment group (NAMPT + local hypothermia).A rat model of local cerebral ischemia-reperfusion was induced by suture method.Infarct volume and cerebral edema volume were assessed by 2,3,5-triphenyltetrazolium chloride staining after 2 h cerebral ischemia and 24 h reperfusion in rats.Evans blue staining was used to assess the extent of blood-brain barrier damage,and a 12-point scale was used to assess neurological deficits.Results The infarct volume in the local hypothermia group,NAMPT group,and combination treatment group was significantly lower than that in the model group (all P ＜0.05).The infarct volume in the combination treatment group was significantly lower than that in the NAMPT group (P ＜0.05).The infarct volume in the combination treatment group was lower than that in the local hypothermia group,but it did not reach statistical significance.The neurological function scores of the local hypothermia group,NAMPT group,and combination treatment group were significantly lower than those of the model group (all P ＜0.05).The score of the combined treatment group was significantly lower than that of the NAMPT group and the local hypothermia group (all P＜0.05).Evans blue leakage in the local hypothermia group,NAMPT group,and combination treatment group was lower than that in the model group (all P ＜0.05),but the differences between each treatment group were not statistically significant.Conclusion NAMPT and local hypothermia combination therapy showed better neuroprotective effects on cerebral ischemia-reperfusion injury,suggesting that the combination therapy had clinical transformation prospects.
