A piglet model of pulmonary vein stenosis
10.7507/1007-4848.201812074
- VernacularTitle:肺静脉狭窄猪模型的建立
- Author:
ZHU Fang
1
,
2
;
WEN Chen
1
,
2
;
SHI Guocheng
1
,
2
;
ZHANG Qian
1
,
2
;
MU Hongwei
1
,
2
;
LIU Gang
1
,
2
;
SHI Bowen
1
,
2
;
YAN Yichen
1
,
2
;
ZHU Zhongqun
1
,
2
;
CHEN Huiwen
1
,
2
Author Information
1. Department of Cardiothoracic Surgery, Shanghai Children&rsquo
2. s Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, P.R.China
- Publication Type:Journal Article
- Keywords:
Pulmonary vein stenosis;
animal testing alternatives;
pulmonary vein;
pathology;
myofibroblast
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2019;26(10):1008-1013
- CountryChina
- Language:Chinese
-
Abstract:
Objective Pulmonary vein banding was used to establish a piglet model of pulmonary vein stenosis. We investigated the pathomorphological alterations of pulmonary veins in the model and compared it with the vascular tissue of recurrent stenosis after total anomalous pulmonary venous connection (TAPVC). Methods Ten pigs of 6 weeks old were selected and randomly divided into 2 groups: 5 in a sham operation group and 5 in a pulmonary vein banding group. The operation had two stages, in which thoracotomies through intercostal space were done respectively on both sides. Biocompatible materials were applied around the pulmonary veins in the experimental group. The same method was used in the sham group. But the pulmonary veins were not banded. Six weeks after the operation, the pulmonary veins of the animals were harvested for hematoxylin-eosin staining and immunofluorescence staining to observe the pathological alterations of pulmonary veins. The proliferative tissues of patients with recurrent stenosis after TAPVC repair were collected and observed by hematoxylin-eosin staining and immunofluorescence staining. Results Both the sham operation group and the pulmonary vein banding group survived. But the pulmonary vein banding group had obvious clinical manifestations of pulmonary venous stenosis. Compared with the sham group, the pulmonary vein banding group showed intimal hyperplasia, decreased expression of endothelial marker and increased expression of mesenchymal markers, and co-expression of endothelial and mesenchymal markers in intimal cells. Human pathology also showed intimal hyperplasia and co-expression of endothelial and mesenchymal markers in intimal cells. Conclusion The surgical pulmonary vein stenosis in piglets shows intimal hyperplasia and myofibroblasts, which was consistent with clinical pathology.
