Major house dust mite allergen, Der p I, activates phospholipase D in human peripheral blood mononuclear cells from allergic patients: involvement of protein kinase C.
- Author:
Jae Won OH
1
;
Jong Hoon KIM
;
Ki Sung LEE
;
Joong Soo HAN
Author Information
1. Department of Pediatrics, College of Medicine, Hanyang University, Seoul, Korea.
- Publication Type:Original Article ; In Vitro ; Research Support, Non-U.S. Gov't
- Keywords:
Der p I;
phospholipase D;
protein kinase C;
allergy;
PBMC
- MeSH:
Adult;
Allergens/metabolism*;
Allergens/immunology;
Animal;
Down-Regulation (Physiology);
Glycoproteins/metabolism*;
Glycoproteins/immunology;
Human;
Hypersensitivity/metabolism;
Hypersensitivity/immunology;
Hypersensitivity/blood;
IgE/blood;
In Vitro;
Leukocytes, Mononuclear/metabolism;
Leukocytes, Mononuclear/immunology;
Mites/metabolism;
Mites/immunology;
Phospholipase D/metabolism*;
Phospholipase D/immunology;
Protein Kinase C/metabolism*;
Skin Tests;
Tetradecanoylphorbol Acetate/pharmacology
- From:Experimental & Molecular Medicine
2000;32(2):67-71
- CountryRepublic of Korea
- Language:English
-
Abstract:
The major house-dust-mite allergen, Der p I, stimulates the phospholipase D (PLD) in peripheral blood mononuclear cells (PBMC) from allergic patients with maximal responses after 30 min exposure. At 30 min, Der p I stimulated PLD activity by 1.4-fold in mild, 1.6-fold in moderate and 2-fold in severe allergic patients over control values (p < 0.05). When the cells were pretreated for 24 h with phorbol myristate acetate to down-regulate protein kinase C (PKC), PLD stimulation by Der p I was largely abolished. These results indicate that in PBMC from allergic patients, Der p I can stimulate PLD activity, and that PKC activation is involved in this stimulation.
