Antioxidant Properties of Stingless Bee Honey and Its Effect on the Viability of Lymphoblastoid Cell Line
- Author:
HAZIRAH H
1
Author Information
1. Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia
- Collective Name:HAZIRAH H; YASMIN ANUM MY; NORWAHIDAH AK
- Publication Type:Journal Article
- Keywords:
antioxidant;
flavonoid;
kelulut honey;
lymphoblastoid cell line;
phenolic;
stingless bee
- From:Medicine and Health
2019;14(1):91-105
- CountryMalaysia
- Language:English
-
Abstract:
Research on the medical benefit of stingless bee honey (kelulut honey) is rather new
although it has been used as traditional food and additive for ages. The primary
92
Med & Health Jun 2019;14(1): 91-105 Hazirah H. et al.
objective of our study was to evaluate the antioxidant properties of kelulut honey
and its effect on lymphoblastoid cell line. We analysed the antioxidant properties
of kelulut honey by ferric reducing antioxidant potential assay, total phenolic and
flavonoid contents using UV spectrophotometry. The total phenolic content, total
flavonoid content and ferric reducing antioxidant potential of Malaysian kelulut
honey produced by Trigona spp. were found to be 844.45 mg RE/kg honey, 78.29
mg RE/kg honey and 1132.66 mM FE/kg honey, respectively. Our findings showed
a strong correlation between total phenolics and flavanoids contents with its
antioxidant potential at R2
= 0.920 and R2
= 0.951, respectively. The effect of honey
on cell viability of lymphoblastoid cell line (LCL) was also investigated. The cells
were cultured in RPMI-1640 medium supplemented with 0 - 500 μg/mL of kelulut
honey for 24 hours. Cell viability was quantitated using 3-(4,5-dimethylthiazol2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, MTS assay
showed that honey supplementation boosted the viability of LCL up to 164.64%
(p< 0.01). The significant increase in cell viability might be modulated by the
antioxidant properties of kelulut honey.
- Full text:12.2019my0107.pdf
