Cell proliferation assessment of human gingival fibroblasts treated with Clinacanthus nutans using alamarBlue assay
- Author:
Pui Szi Kelvin Yong
1
Author Information
1. a School of Dental Sciences, Universiti Sains Malaysia 16150 Kubang Kerian, Kelantan, Malaysia.
- Collective Name:Wan Afiqah Syahirah Wan Ghazali; Siti Fadilah Abdullah; Norzaliana Zawawi; Thirumulu Ponnuraj Kannan
- Publication Type:Journal Article
- Keywords:
AlamarBlue assay;
cell proliferation;
Clinacanthus nutans;
human gingival fibroblast;
Sabah snake grass.
- From:Archives of Orofacial Sciences
2018;13(2):80-85
- CountryMalaysia
- Language:English
-
Abstract:
Clinacanthus nutans (C. nutans), a well-known ethnopharmacological plant consumed for its medicinal
purposes by Southeast Asian communities. C. nutans is said to possess antipyretic, inflammatory, antiedemic as
well as analgesic properties and used traditionally in treating various skin ailments, Herpes infection, cancer and
diabetes. The young leaves of this C. nutans are consumed in Malaysia for maintaining health. In this study, the
proliferative activity of human gingival fibroblast cells (HGF-1, ATCC®CRL-2014™, USA) treated with the ethanol
extract obtained from C. nutans leaves at three different concentrations (250, 125 and 62.5 µg/ml) was compared
with the untreated cells using alamarBlue assay. The proliferative activity of HGF-1 using alamarBlue assay
showed that the cells treated with 62.5 μg/ml of ethanolic extract of C. nutans leaves exhibited increased
proliferation compared to the other groups and hence does not exhibit any cytotoxicity on HGF-1.
- Full text:1.2018my01581.pdf