Research on the inhibitory effect of Baicalin on the human tongue squamous cell carcinoma cell line SCC15
10.12016/j.issn.2096-1456.2019.04.004
- Author:
ZHONG Wende
1
;
LIU Rongjing
2
;
GUAN Hongbing
3
;
CHEN Guangsheng
1
Author Information
1. Department of Stomatology, the Second Affiliated Hospital of Guangzhou Medical University
2. Department of Clinical Laboratory, the Second Affiliated Hospital of Guangzhou Medical University
3. Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Diseases, Affiliated Stomatological Hospital to Guangzhou Medical University
- Publication Type:Journal Article
- Keywords:
Baicalin;
Tongue;
Carcinoma;
Squamous cell;
SCC15 cell;
Migration;
Cell cycle;
Signal transduction and transcription activator 3(STAT3)
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2019;27(4):226-230
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the inhibitory effect and possible mechanism of Baicalin on the human tongue squamous cell carcinoma cell line SCC15 and to provide a new idea and experimental basis for the clinical prevention and treatment of tongue squamous cell carcinoma.
Methods :SCC15 cells cultured in DMEM alone were used as the control group, and SCC15 cells cultured in 20 mg/mL baicalin solution were used as the baicalin group. Scratch tests and Transwell migration tests were performed to detect changes in cell migration ability, and flow cytometry was used to detect changes in the cell cycle. Western blotting was used to detect differences in the phosphorylation levels of signal transduction and transcription activator 3 (STAT3).
Results : Compared with the control group, the scratch test and the Transwell migration test showed that the cell migration ability of cells in the baicalin group was significantly decreased (t=4.927, P=0.008); flow cytometry showed that the number of cells of the baicalin group increased in the G0/G1 phase (t=9.893, P=0.001), decreased in the S phase (t=8.528, P=0.001), and decreased in the G2/M phase (t=3.550, P=0.024); Western blotting results showed that the STAT3 protein of SCC15 cells in the baicalin group decreased (t=3.550, P=0.024), and the phosphorylation level significantly decreased (t=8.262, P=0.001).
Conclusion :Baicalin inhibits the human tongue squamous cell carcinoma cell line SCC15, and its mechanism may be related to a decrease in STAT3 pathway phosphorylation activity.
- Full text:黄芩苷对人舌鳞癌细胞SCC15的抑制作用1.pdf
