Acute Toxicity Study of Intravenous Administration of Thymoquinone-Loaded Nanostructured Lipid Carrier (TQ-NLC) in Sprague Dawley Rats
- Author:
Latifah Saiful Yazan
1
Author Information
1. Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
- Collective Name:Latifah Saiful Yazan; Siti Nabilahuda Mohd Azlan; Fatin Hannani Zakarial Ansar; Banulata Gopalsamy
- Publication Type:Journal Article
- Keywords:
Thymoquinone;
Intravenous administration;
Nanostructured lipid carrier;
Acute toxicity
- MeSH:
Acute toxicity
- From:Malaysian Journal of Medicine and Health Sciences
2019;15(SP2):51-57
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Thymoquinone (TQ), a bioactive compound from Nigella sativa is known for its various medicinal properties. Due to the low solubility of TQ, nanostructured lipid carrier (NLC) has been used as a delivery system to improve its efficacy. Nevertheless, the effect of TQ-NLC when administered intravenously is unclear. This study investigated the acute toxicity profile of intravenous administration of TQ-NLC in an in vivo model. Methods: Twelve female Sprague dawley rats were assigned randomly into two groups (n=6); a control and a treatment group that received normal saline and 25 mg/kg TQ-NLC, respectively, via intravenous injection. The rats were observed for 14 days for any alterations to their usual physical conditions such as behaviour and mortality, body weight, food intake, organ-to-body weight ratio, and haematological, biochemical and histopathological profile. Results: There were no significant changes (p>0.05) in the body weight, food intake, organ-to-body weight ratio, and haematological, biochemical and histopathological profile between TQ-NLC treatment and the control group. However, inflammation was observed at the site of injection on the rat’s tail. Conclusion: Intravenous administration of TQ-NLC (25 mg/kg) did not exert acute toxic effect in female Sprague dawley rats. The data can be used as a basis to further develop TQNLC as a potential therapeutic drug.
- Full text:11.2019my0430.pdf
