A preliminary study of platelet hyperactivity in the chronic indeterminate phase of Chagas’ disease
- Author:
Flavio Rojas Castillejos
1
;
Laura Perez-Campos Mayoral
2
;
Gabriel Mayoral Andrade
2
;
Maria Teresa Hernandez-Huerta
2
;
Socorro Pina-Canseco
2
;
Ruth Martinez Cruz
2
;
Efrain Herrera Colmenares
2
;
Eduardo Perez-Campos Mayoral
2
;
Paz Maria Salazar
3
;
Martha Bucio Torres
3
;
Margarita Cabrera Bravo
3
;
Margarito Martinez Cruz
4
;
Carlos Matí
;
as Cervantes
4
;
Roxana Diaz Albarraz
1
;
Joel Lopez Matias
1
;
Gabriela Ines Rios Arias
1
;
Gema Hrnandez Bernardino
1
;
Ernesto Perez Matus
1
;
Rosalinda Mendez Trujillo
1
;
Luis Manuel Sanchez Navarro
2
;
Alma Dolores Perez Santiago
4
;
Eduardo Perez Campos
2
,
4
,
5
,
6
Author Information
- Publication Type:Journal Article
- From:Tropical Biomedicine 2018;35(3):678-683
- CountryMalaysia
- Language:English
- Abstract: The chronic indeterminate phase of Chagas’ disease is asymptomatic despite positive test results for antibodies specific to Trypanosoma cruzi. CD62P-APC (P-selectin) and PAC-1 FITC (GpIIb/IIIa) may improve diagnosis as biomarkers of platelet activity. Nine asymptomatic seropositive subjects, previously untreated, were selected from a blood bank within a year of Chagas’ disease detection, in addition to a control group of four. All subjects were evaluated by flow cytometry for CD62P, PAC-1 and CD41, and in a complementary study, by Tissue Doppler Echocardiography for isovolumic relaxation times (IVRT) and E/A ratios. The subjects were classified as positive or negative for CD62P and PAC-1 by a cut off obtained from their mean±2SD. For IVRT and E/A ratios, cut offs were obtained from the American Society of Echocardiography and the European Association of Cardiovascular Imaging recommendations. Fisher’s exact test was used for associated findings. Pre-test and post-test probability, sensitivity, specificity, positive and negative predictive values and likelihood ratios were calculated. Abnormalities were expressed as platelet hyperactivity and ventricular dysfunction in CD62P, PAC-1, IVRT and E/A ratios. CD62P appears to have greater sensitivity (0.75) and PAC-1, more accurate specificity (0.75), which may explain thrombotic events in Chagas’ disease. We recommend the use of CD62P and PAC-1 as biomarkers of platelet hyperactivity in patients in the chronic indeterminate phase of Chagas’ disease.
- Full text:8.2018my0941.pdf
