Indirect Immunofluorescence Screening of Potential Arthritic Autoantibodies in Systemic Lupus Erythematosus
- Author:
Malarvili Selvaraja
1
,
2
Author Information
1. Department of Medical Microbiology &
2. Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
- Collective Name:Malarvili Selvaraja; Che Ku Dahlan Che Ku Daud; Marsitah Abdul Jalil; Anim Md. Shah; Syafinaz Amin Nordin; Zamzarina Ahmad Bajari; Chong Chou Min; Maha Abdullah
- Publication Type:Journal Article
- Keywords:
Autoantibodies;
Lupus arthritis;
Immunofluorescence
- MeSH:
Lupus arthritis
- From:Malaysian Journal of Medicine and Health Sciences
2018;14(SP3):41-44
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Joint involvement is common in systemic lupus erythematosus (SLE) patients, however, screening for joint specific autoantibodies in patients is not routinely performed. This may be due to the lack of known antigens and available tissue. The rat musculoskeletal tissue may be a suitable source of antigen to detect arthritic autoantibodies. Method: We tested plasma of SLE patients, with arthritis (N=9) and without arthritis (N=7) as well as plasma from normal individuals (N=7) on fresh sectioned tissue from rat plantar hind paw using indirect immunofluorescence method. Results: Binding of autoantibodies to striation in skeletal muscle cells in the tissue was clearly demonstrable in all samples from SLE with arthritis but not on slides incubated with plasma from normal or SLE without arthritis. Conclusion: Thus, rat plantar tissue may be suitable for detecting autoantibodies from SLE patients that may be involved in the pathogenesis of lupus arthritis.
- Full text:11.2018my0302.pdf
