Effects of Porphyromonas gingivalis infection with different fimA genotypes on the secretion of IL-1β, IL-6, and TNF-α by human umbilical vein endothelial cells
10.12016/j.issn.2096-1456.2019.06.005
- Author:
CAI Shuyu
1
,
2
;
LIN Xiaoyue
2
,
3
;
LEI Jin
1
,
2
;
GE Song
4
Author Information
1. Department of Stomatology, Affiliated Dongguan Hospital of Medical College of Jinan University, The Fifth People&prime
2. s Hospital of Dongguan
3. Department of Endocrinology, Affiliated Dongguan Hospital of Medical College of Jinan University, The Fifth People&prime
4. Affiliated Stomatological Hospital of Zunyi Medical University
- Publication Type:Journal Article
- Keywords:
Porphyromonas gingivalis;
Interleukin;
Tumor necrosis factor-α Atherosclerosis;
Periodontosis
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2019;27(6):364-369
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of Porphyromonas gingivalis (P. g) infection with I, II and IV fimA genotypes on the expression of IL-1beta, IL-6 and TNF-alpha in human umbilical vein endothelial cells (HUVECs).
Methods :HUVECs infected with different fimA genotypes were divided into the fimA type I stimulation group, fimA type II stimulation group, fimA type IV stimulation group. In addition, a positive control group (E. coli LPS stimulation) and negative control group (cell culture medium only) were included. Cell proliferation was detected by MTT assay, and cell apoptosis was detected by flow cytometry. IL-1β, IL-6, TNF-α levels in the supernatant of HUVECs after P. g stimulation were assessed by ELISA at 2 h, 6 h and 24 h.
Results :HUVECs were infected by P. g with fimA type I,fimA type II and LPS for 24 h. Cell proliferation was inhibited compared with the negative control group (P < 0.05), but there was no significant difference in the apoptosis rate between P. g infection and the negative control group. IL-1β levels in cell culture supernatants were higher at 2th than 6 and 24 h after stimulation of HUVECs with different fimA genotypes, while the IL-6 levels were higher at 24 h than the other time, while the TNF-α levels were no significant difference at every time. After fimA type II and IV P. g infection, IL-1β, IL-6, and TNF-α levels were increased compared with fimA type I P. g (P < 0.05).
Conclusion : Different P. g fimA genotypes have different effects on stimulating HUVECs to induce dysfunction. Here, fimA type II and IV P. g exhibit a strong ability to upregulate the secretion of IL-1β, IL-6 and TNF-alpha.
- Full text:不同fimA基因型牙龈卟啉单胞菌感染对人脐静脉内皮细胞分泌IL-1β、IL-6、TNF-α的影响.pdf
