Characterisation of immunogenotypes of diffuse large B-cell lymphoma
- Author:
Phang Kean Chang
1
Author Information
1. Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia
- Collective Name:Kean Chang Phang; Noor Hamidah Hussin; Faridah Abdul Rahman; Nur Maya Sabrina Tizen; Adnan Mansoor; Noraidah Masir
- Publication Type:Journal Article
- Keywords:
diffuse large B-cell lymphoma;
immunohistochemistry;
fluorescent in situ hybridization;
gene rearrangement;
Malaysia
- MeSH:
immunohistochemistry
- From:The Malaysian Journal of Pathology
2019;41(2):101-124
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Diffuse large B-cell lymphoma (DLBCL) is the most common aggressive type of non-Hodgkin lymphoma with variable clinical outcomes. The immunogenotypic features of this heterogeneous disease in Malaysia were not well characterized. Materials & Methods: In total 141 local series of DLBCL cases from UKM Medical Centre were retrospectively studied. Results: Of these cases, we classified our patients into two subtypes: 32.7% (37/113) GCB and non-GCB 67.3% (76/113) by Hans algorithm and the results showed strong agreement with the results by Choi algorithm (κ = 0.828, P<0.001). Survival analysis indicated significant difference in between GCB and non-GCB subtypes (P=0.01), elevated serum LDH (P=0.016), age more than 60-year-old (P=0.021) and the presence of B symptoms (P=0.04). We observed 12% DLBCL cases were CD5 positive and 81.8% of them died of the disease (P=0.076). Analysis on the dual expression of MYC/ BCL2 revealed that there is no significant difference in DE and non-DE groups (P=0.916). FISH study reported there were 9.22% (13/141) rearranged cases observed in our population at which highest frequency of BCL6 gene rearrangement (76.9%), followed by MYC (15.4%) and BCL2 (7.7%); no BCL10 and MALT-1 gene rearrangement found regardless of using TMAs or whole tissue samples. More cases of MYC protein overexpression observed compared to MYC translocation. Conclusion: Relatively lower frequency of GCB tumours and low gene rearrangement rates were observed in Malaysian population. A national study is therefore warranted to know better the immunogenotypic characteristics of DLBCL in Malaysia and their implications on the survival.
- Full text:5.2019my1001.pdf
