The differential roles of caspase family members in mediating PF4-induced breast cancer apoptosis
- Author:
Tengku Ahmad Damitri Al Astani Tengku Din
1
Author Information
1. Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Malaysia
- Collective Name:Tengku Ahmad Damitri Al Astani Tengku Din; Muhammad Irfan Abdul Jalal; Azman Seeni; Shaharum Shamsuddin; Hasnan Jaafar
- Publication Type:Journal Article
- Keywords:
PF4;
breast cancer;
caspase;
apoptosis
- MeSH:
breast cancer
- From:The Malaysian Journal of Pathology
2018;40(3):303-312
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: This study focused on PF4 effects on caspase-3,-6, -7, -8 and -9 which regulate the apopotosis process in breast cancer. Materials and Methods: Breast tumours were induced in forty 21-day-old female Sprague Dawley rats (SDRs) using MNU until tumour size reached 14.5 mm (SD: 0.5 mm). The rats were then divided into two groups: Group 1 (control injected with 0.9% saline; n = 20), and Group 2 (platelet factor 4 (PF4); n = 20). PF4 was administered through focal intralesional injection at 20 µg/lesion dose. Following 5-day treatment, the SDRs were sacrificed. Subsequently, representative sections from the tumour were obtained for haematoxylin and eosin (H&E) staining. The expressions of caspase-3, -6, -7, -8 and -9 were evaluated using immunohistochemistry (IHC) staining. Results: The majority of breast tumour specimens were of aggressive types [ncontrol = 13 (65%); nPF4 = 12 (60%)]. Invasive ductal carcinoma not otherwise specified (IDC-NOS) was the most commonly observed breast tumour histology for control and PF4 groups (n = 8 (40%) in respective groups). PF4-treated group exhibited significant differences in the caspase-3, -6 and -8 expression levels compared to the control group (all p < 0.001). There were no significant differences in caspase-7 (p = 0.347) and caspase-9 (p = 0.373) expression levels between both groups. Conclusion: This study found that PF4 acts via the caspase-mediated extrinsic apoptosis pathway without the involvement of the intrinsic pathway.
- Full text:5.2018my0975.pdf
