Detection of human papillomavirus types 16 and 18 in oral squamous cell carcinoma samples in Malaysia
- Author:
Fatin Hazwani Fauzi
1
Author Information
1. School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
- Collective Name:Nurul Izzati Hamzan; Nurhayu Ab. Rahman; Irfan Mohamad; Siti Suraiya; Thomas George Kallarakkal; Suharni Mohamad
- Publication Type:Journal Article
- Keywords:
HPV;
nested PCR;
OSCC;
saliva;
tissue.
- From:Archives of Orofacial Sciences
2019;14(1):21-29
- CountryMalaysia
- Language:English
-
Abstract:
Infection of the oral cavity with high-risk human papillomavirus (HPV) has been implicated as one of the
risk factors for the development of oral squamous cell carcinoma (OSCC). Among the high-risk HPV types, HPV
16 and 18 are the most common infective agents in oral cancers. This study aimed to compare the presence of
high-risk HPV in genetic materials obtained from saliva, blood and tissues of OSCC patients in Malaysia. The
genomic DNA was extracted from saliva (n=13), blood (n=59) and tissue (n=63) and subjected to polymerase chain
reaction (PCR) amplification of human beta globin gene to confirm the presence and integrity of DNA. Positive
amplification was then screened for high-risk HPV by nested PCR using MY11/09 and GP5+/6+ consensus
primers, followed by a further confirmation by DNA sequencing of the positive samples. As a result, two saliva
samples (2/13; 15.4%) were found to harbour HPV 16 and one tissue sample (1/63; 1.6%) was shown to be positive
for HPV 18. However, none of the blood samples were positive for high-risk HPV. Thus, HPV is more likely to be
found in the saliva of OSCC patients as compared to blood and tissue samples. The detection of high-risk HPV in
OSCC patients is useful in deciding how to manage the patient as HPV-associated OSCC has better prognosis.
- Full text:1.2019my0168.pdf
