Early morphology observation of human periodontal fibroblast cell line in the presence of human salivary exosomes
- Author:
Tuan Siti Mastazliha Long Tuan Kechik
1
Author Information
1. School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
- Collective Name:Zurairah Berahim; Wan Nazatul Shima Shahidan
- Publication Type:Journal Article
- Keywords:
Cell morphology;
human periodontal ligament fibroblast cell;
human salivary exosomes;
scanning electron microscopy
- From:Archives of Orofacial Sciences
2019;14(1):11-20
- CountryMalaysia
- Language:English
-
Abstract:
Human salivary exosomes have been identified as a highly informative nanovesicle with clinical-relevant
information for variation of diagnostic purposes. As a continued effort from previous studies on human salivary
exosomes effect at gene expression level, this study is carried out to observe the morphology of human periodontal
fibroblast (HPdLF) treated with exosomes cells under the same period of changes in genotypic level occurred. In
vitro, HPdLF cells were cultured for 24 hours with 10 µg/ml of human salivary exosomes. The morphology of HPdLF
cells was examined under inverted light microscopy and scanning electron microscopy (SEM) for both control
samples and samples treated with human salivary exosomes, while the cell count was performed via trypan blue
staining. There was no significant difference in the morphology under the inverted light microscopy and the cell
number of HPdLF cells for both treated and untreated cells with exosomes. However, for SEM, the treated HPdLF
with salivary exosomes showed slight observable changes on the filopodia, lamellipodia, cytoplasmic vesicles and
the cytoskeleton of the cells. Even within a short period (24 hours) of culturing time for cells with human salivary
exosomes, the samples showed minimal changes which positively suggested a simultaneous event of exchanging
materials from human salivary exosomes to cells had occurred, hence, potentially proving that human salivary
exosomes can enhance cell proliferation
- Full text:1.2019my0167.pdf
