Inhibitory effect and mechanism of epigallocatechin-3-gaUate on autogenous vein graft stenosis in rat models
10.7507/1007-4848.201704044
- VernacularTitle:表没食子儿茶素-3-没食子酸(EGCG)对大鼠自体移植静脉桥狭窄的抑制作用及其机制研究
- Author:
ZHANG Yi
1
;
GU Jun
2
;
LIU Linbo
1
;
LIAO Zhijie
1
;
ZHANG Hongwei
2
;
YANG Peng
2
;
FAN Kangjun
2
;
LIANG Huaimin
2
;
XIAO Zhenghua
2
;
HU Jia
2
Author Information
1. Department of Cardiovascular Surgery, Third Hospital of Mianyang, Mianyang, 621000, Sichuan, P.R.China
2. Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, 610041, P.R.China
- Publication Type:Journal Article
- Keywords:
Vein grafts;
epigallocatechin-3-gallate;
Notch signal pathway;
HES1
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2017;24(10):791-790
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of epigallocatechin-3-gallate (EGCG) on restenosis of the vein graft. Methods Totally 90 Sprague-Dawley rats were randomly divided a the control group, a vein graft group and an EGCG+vein graft group. At week 1, 2 and 4, the intimal and tunica thickness of the venous graft wall was evaluated by hematoxylin-eosin staining, and the expression of Ki-67 was assessed by immunohistochemistry analysis, and then the expression of hairy and enhancer of split-1 (HES1) was measured by Western blot assay. Results At week 2, the intimal thickness (46.76±4.89 μm vs. 8.93±0.82 μm, 46.76±4.89 μm vs. 34.24±3.57 μm), tunica thickness (47.28±4.37 vs. 16.33±1.52 μm, 47.28±4.37 vs. 36.27±3.29 μm), positive cell rate of Ki-67 (21.59%±2.29% vs. 1.12%±0.22%, 21.59%±2.29%vs. 15.38%±1.30%), expression of HES1 respectively increased in the experimental group than those in the control group and the EGCG+vein graft group (P<0.05, respectively). At week 4, the intimal thickness (66.38±6.23 μm vs. 8.29±0.79 μm, 66.38±6.23 μm vs. 48.39±4.23 μm), tunica thickness (63.27±6.18 μm vs. 15.29±1.49 μm, 63.27±6.18 μm vs. 44.63±4.49 μm), positive cell rate of Ki-67 (33.19%±3.03% vs. 1.09%±0.19%, 33.19%±3.03% vs. 24.37%±2.73%), expression of HES1 increased in the experimental group than those in the control group and EGCG+vein graft group (P<0.05, respectively). Conclusion EGCG may inhibite restenosis of vein graft by inhibiting Notch signal pathway.
