Comparison of nasal cytokine profiles of human metapneumovirus and respiratory syncytial virus
10.5415/apallergy.2017.7.4.206
- Author:
Jin Sung PARK
1
;
Young Ho KIM
;
Eunmi KWON
;
Zak CALLAWAY
;
Takao FUJISAWA
;
Chang Keun KIM
Author Information
1. Asthma and Allergy Center, Department of Pediatrics, Inje University Sanggye Paik Hospital, Seoul 01757, Korea. kimck@paik.ac.kr
- Publication Type:Original Article
- Keywords:
Child;
Cytokines;
Metapneumovirus;
Respiratory syncytial virus;
Wheezing
- MeSH:
Child;
Cytokines;
Humans;
Inflammation;
Interleukin-2;
Interleukin-4;
Interleukin-6;
Metapneumovirus;
Respiratory Sounds;
Respiratory Syncytial Viruses;
Respiratory Tract Infections
- From:
Asia Pacific Allergy
2017;7(4):206-212
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Human metapneumovirus (hMPV) and respiratory syncytial virus (RSV) share some epidemiological and clinical characteristics; however, few studies have examined the mechanisms by which these viruses induce airway inflammation. OBJECTIVE: This study was undertaken to compare cytokine profiles in hMPV and RSV patients to investigate possible differences in inflammatory pathways. METHODS: Nasopharyngeal aspirate specimens were collected from 1,008 pediatric patients hospitalized for acute lower respiratory tract infection with wheezing and 20 normal healthy controls. Patients were tested for 7 common respiratory viruses then divided into hMPV (n = 35) and RSV groups (n = 67). T helper (Th) 1 (interferon [IFN]-γ), Th2 (interleukin [IL]-4, eotaxin) and Th17 (IL-1β, IL-6) cytokine profiles were analyzed in the 3 groups. RESULTS: IFN-γ and IL-2 levels were significantly increased in the hMPV and RSV groups compared to the control group (p < 0.0001 and p < 0.0001, respectively). IL-4 levels were significantly higher in the RSV group compared to the hMPV and control groups (p = 0.0003 and p < 0.0001, respectively). Eotaxin levels showed a tendency to be higher in the RSV group compared to the hMPV group (p = 0.0580), and significantly higher compared to the control group (p < 0.0001). IL-1β levels were significantly higher in the hMPV compared to the RSV group (p < 0.0001), and IL-6 levels were significantly higher in the hMPV group compared to the control group (p < 0.0001). CONCLUSION: Our results suggest that hMPV and RSV have different inflammatory mechanisms. hMPV induces airway inflammation by the Th17 pathway through release of IL-1β and IL-6, whereas RSV acts through the Th2 pathway.
