Measurement of Hymenoptera venom specific IgE by the IMMULITE 3gAllergy in subjects with negative or positive results by ImmunoCAP
10.5415/apallergy.2012.2.3.195
- Author:
Mineaki WATANABE
1
;
Hirokuni HIRATA
;
Masafumi ARIMA
;
Yumeko HAYASHI
;
Kazuyuki CHIBANA
;
Naruo YOSHIDA
;
Yoshihiko IKENO
;
Yasutsugu FUKUSHIMA
;
Reiko KOMURA
;
Kazumi OKAZAKI
;
Kumiya SUGIYAMA
;
Takeshi FUKUDA
Author Information
1. Department of Pulmonary Medicine and Clinical Immunology, Dokkyo University School of Medicine, Tochigi 321-0293, Japan. hirokuni@dokkyomed.ac.jp
- Publication Type:Original Article
- Keywords:
Allergy;
IMMULITE;
ImmunoCAP;
Sensitivity;
Specificity;
Venom specific IgE
- MeSH:
Bites and Stings;
Diagnosis;
Humans;
Hymenoptera;
Hypersensitivity;
Immunoglobulin E;
Limit of Detection;
Methods;
Sensitivity and Specificity;
Skin;
Venoms;
Wasps
- From:
Asia Pacific Allergy
2012;2(3):195-202
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Patients may receive negative results from a specific IgE (sIgE) test such as the ImmunoCAP (CAP) despite a documented history of systemic reaction to a Hymenoptera sting. Thus, further testing may be required using another serological method or venom skin prick tests to confirm allergy diagnosis and correct species. OBJECTIVE: To evaluate the sensitivity and the specificity of CAP and IMMULITE 3gAllergy (IMMULITE) for detecting sIgE to Paper wasp (WA) and Yellow Jacket (YJ) venoms using patient clinical history as the comparator. METHODS: Sera from 70 participants with a history of systemic reactions (SR) to WA and/or YJ stings were tested using CAP and IMMULITE. Fifty participants from this group had negative results on CAP. To assess specificity, sera from 71 participants who had never experienced either a WA or YJ sting were tested using CAP and IMMULITE. Fifty participants from this group tested positive using CAP. RESULTS: In participants with a history of systemic reaction to a Hymenoptera sting, yet who tested negative for WA and/or YJ sIgE according to CAP, the positivity rate according to IMMULITE was 20-42% using 0.10 IUA/mL as the limit of detection (LoD), per the manufacturer's specification. When the LoD for CAP (0.35 IUA/mL) was applied to the IMMULITE results, positivity according to IMMULITE was 14-26%. Overall, sensitivity, specificity, and agreement with SR were greater for IMMULITE than for CAP. For YJ: sensitivity (IMMULITE:CAP), 42.8%:28.5%; specificity, 53.5%:39.4%; agreement, 48.2%:34%. For WA, sensitivity (IMMULITE:CAP), 58.6%:28.5%; specificity, 49.3%:47.8%; agreement, 43.9%:38.3%. CONCLUSION: The IMMULITE performed well for detecting sIgE to Hymenoptera venom
