Differential expression of BKCa channels in atrial fibroblasts in patients with sinus rhythm and atrial fibrillation
10.7507/1007-4848.201707042
- VernacularTitle:窦性心律与心房颤动患者心房成纤维细胞大电导钙激活钾通道表达差异研究
- Author:
BI Hongqin
1
;
LI Miaoling
2
;
ZHOU Wei
1
;
LI Guang
2
;
DONG Yahui
1
;
HUANG Tao
1
;
YU Fengxu
1
Author Information
1. Department of Cardiothoracic Surgery, the Affiliated Hospital of Southwest Medical University, Luzhou, 646000, Sichuan, P.R.China
2. The Key Laboratory of Medical Electrophysiology, Ministry of Education of China, Southwest Medical University, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease of Sichuan Province, Luzhou, 646000, Sichuan, P.R.China
- Publication Type:Journal Article
- Keywords:
Atrial fibrillation;
sinus rhythm;
fibroblasts;
BKCa
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2017;24(12):937-942
- CountryChina
- Language:Chinese
-
Abstract:
Objective Through analyzing BKCa channel expression in atrial fibroblasts in patients with sinus rhythm and atrial fibrillation (AF), to explore the mechanism of myocardial fibrosis and provide new therapeutic strategies for the treatment and reversal of AF structure reconstruction. Methods We selected 10 patients of rheumatic heart valvular disease who underwent valve replacement surgery. They were 5 patients with sinus rhythm (a sinus rhythm group, 2 males and 3 females with an average age of 49.1±8.3 years) and 5 with AF (an AF group, 3 males and 2 females with an average age of 50.3±5.8 years). About 100 mg tissue was obtained from the right auricula dextra, and the atrial fibroblasts were cultured by tissue block adherence method, and the expression of BKCa channel genes and proteins in cultured fibroblasts was detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting methods. Results (1) The general data of 10 patients between the AF group and the sinus rhythm group were compared. There was no significant difference between the two groups in age (t=1.21, P=0.67) and sex (t=2.56, P=0.75). There was statistical difference in the left atrial diameter and the right atrium diameter between the two groups (t=19.45, P=0.01; t=23.52, P=0.06); (2) the mRNA expression of BKCa subunit was detected by qRT-PCR method, and there was no significant difference in the mRNA expression of BKCa α and BKCa β1 between the two groups (t=3.14, P=0.79; t=2.88, P=0.69); (3) the expression of BKCa protein was detected by western blotting method, and there was no significant difference in the protein expression of BKCa α and BKCa β1 between the two groups (t=0.55, P=0.31; t=0.73, P=0.46). Conclusion BKCa pathway may not be involved in the pathogenesis and maintenance of AF, but it may play an important role in the process of myocardial fibrosis.
