Role of kinase insert domain containing receptor (KDR) positive cells in formation of cardiospheric structure, myocardium and vessels
10.7507/1007-4848.201701025
- VernacularTitle:血管内皮生长因子受体（KDR）阳性细胞在心肌球样结构、血管和心肌成形中的作用
- Author:
LI Li
1
;
ZHANG Weiran
1
;
JIANG Xiaogang
1
;
HAN Zhifeng
1
;
LIU Chao
1
;
LIU Lei
1
;
WANG Xiaoming
1
Author Information
1. Department of Cardiothoracic Surgery, BenQ Medical Center, the Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, 210019, P.R.China
- Publication Type:Journal Article
- Keywords:
Cardiospheric structure;
kinase insert domain containing receptor;
cardiogenesis;
vasculogenesis;
cardiosphere-derived cells
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2018;25(4):333-337
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of kinase insert domain containing receptor (KDR) positive cells in the formation of cardiospheric structure, myocardium and vessels. Methods Twenty-four Wistar rats weighting 250 g were selected. Cardiosphere-derived cells were isolated by enzymatic digestion of rat hearts, and their immunological phenotypes were analyzed by using fluorescence-activated cell sorting (FACS). The cardiomyogenic and vasculogenic potential was diagnosed by immunohistochemistry. Results KDR positive cells grew exponentially and formed cell clusters. It also could generate myocardial precursor cells (cardiac troponin T positive). And these cells can develop spontaneous contraction activity in vitro. Meanwhile, KDR positive cells formed many vessel-like structures through a budding process. Conclusion KDR positive cells form cardiospheric structure in vitro culture, and exhibit differentiation potential towards the cardiac and vascular cells. Therefore, KDR positive cells may have a broad prospect of clinical application as cell donors.
