Expression and clinical significance of MICA in laryngeal carcinoma tissue and cells.
- Author:
Rui WANG
;
Junfu WANG
;
Qinghong SU
;
Xuemei CHEN
;
Xiaoqun XU
- Publication Type:Journal Article
- MeSH:
Carcinoma, Squamous Cell;
metabolism;
pathology;
Cell Line, Tumor;
Head and Neck Neoplasms;
metabolism;
pathology;
Histocompatibility Antigens Class I;
metabolism;
Humans;
Laryngeal Neoplasms;
metabolism;
pathology;
Squamous Cell Carcinoma of Head and Neck
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2016;30(2):94-97
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the mRNA and protein expression of MICA in laryngeal squamous cell carcinoma tissue and the Hep-2 cells.
METHOD:Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western-blot were used to detect the expression of MICA mRNA and protein levels in the Hep-2 cells and laryngeal cancer tissues.
RESULT:The MICA mRNA showed higher expression in Hep-2 cells by RT-PCR. Compared with the control, the mRNA expression of MICA was significantly enhanced in laryngeal cancer tissues (t = 11.878, P < 0.01). The intensity of MICA expression is not related to the clinical stage of cancer. MICA protein demonstrated higher level expression by Western blot. The intensity of MICA protein expression was decreased with increased clinical stage in laryngeal cancer tissues.
CONCLUSION:The MICA mRNA showed stronger expression in Hep-2 cells and laryngeal cancer tissues. The intensity of its expression is not related to clinical stage of cancer. The MICA protein expression was strong in Hep-2 cells. The intensity of MICA protein expression was decreased with increased clinical stage in laryngeal cancer tissues. MICA may play an important role in laryngeal carcinoma process.
