Expression and significance of p-Stat3 and its downstream gene c-myc in laryngeal squamous cell carcinoma tissue and cell line.
- Author:
Li LIU
;
Ying WANG
;
Jianzhong SANG
- Publication Type:Journal Article
- MeSH:
Carcinoma, Squamous Cell;
metabolism;
pathology;
Cell Line, Tumor;
Head and Neck Neoplasms;
metabolism;
pathology;
Humans;
Immunohistochemistry;
Laryngeal Neoplasms;
metabolism;
pathology;
Phosphorylation;
Proto-Oncogene Proteins c-myc;
metabolism;
STAT3 Transcription Factor;
metabolism;
Signal Transduction;
Squamous Cell Carcinoma of Head and Neck
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2016;30(2):123-129
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To detect the expressions of p-Stat3 and c-myc in human laryngeal squamous cell carcinoma (LSCC) tissue and Hep2 cell line, and to find the relationship between them.
METHOD:Immunohistochemistry was used to detect the expressions of p-Stat3 and c-myc in 60 cases of LSCC and 30 cases of vocal cord polyp tissue. The protein levels of p-Stat3 and c-myc in Hep2 cell line was determined by immunocytochemistry. Western blotting was used to determine the protein levels of p-Stat3 and c-myc in Hep2 after treating with different concentrations of Stattic.
RESULT:The positive rates of p-Stat3 and c-myc were 65% and 70% in the LSCC tissue, compared with that in the vocal cord polyp tissue, with significant difference (P < 0.05). The expression of p-Stat3 in LSCC tissue was associated with that of c-myc (r = 0.273, P < 0.05). The protein levels of p-Stat3 and c-myc were detected in the Hep2 cell line. Stattic inhibited Stat3 phosphorylation and c-myc in the Hep2 cell line in a concentration-dependent manner.
CONCLUSION:p-Stat and c-myc were up regulated in the tissue of laryngeal squamous cell carcinoma and the Hep2 cell line. Stattic inhibits the constitutively active p-Stat3 signaling pathway, and downregulats the expression of c-myc. The strong constitutive p-Stat3 signaling pathway in LSCC makes p-Stat3 a target for the development of novel therapeutic strategies.
