Effects of IAA/HRP on the proliferation and apoptosis of SACC-83 cells.
- Author:
Jilian LV
;
Ning GAO
;
Wei HE
- Publication Type:Journal Article
- MeSH:
Adaptor Proteins, Signal Transducing;
metabolism;
Apoptosis;
drug effects;
Caspase 3;
metabolism;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Humans;
Indoleacetic Acids;
pharmacology;
Inhibitor of Apoptosis Proteins;
metabolism;
Neoplasm Proteins;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2016;30(1):31-34
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effect of IAA/HRP on the proliferation and apoptosis of human SACC-83 cells in vitro,and its molecular mechanism.
METHOD:The rate of proliferation inhibition was determined by CCK-8 assay, the apoptosis-related gene Caspase-3 and Livinα mRNA and protein expression levels were measured by real-time PCR and Western blot.
RESULT:IAA/HRP could inhibit proliferation of SACC-83, which depending on the time and dosage (P < 0.05); can-up-regulate the Caspase-3 mRNA and protein expression levels and reduction of the mRNA and protein of the Livina expression, (both P < 0.05).
CONCLUSION:IAA/HRP can inhibit the proliferation and induce the apoptosis of SACC-83 cells, which may due to its regulation of the expression of Caspase-3 and Livinα mRNA expression.
