Effectiveness of cisplatin on the expressions of Bcl-2 and Bax in cochlea and spiral ganglion cells of guinea pigs.
- Author:
Peng LI
;
Wei ZHANG
;
Yi ZHANG
;
Qiuyang FU
;
Qingxiang MENG
;
Jinghua XIE
;
Yong LIANG
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Cisplatin;
pharmacology;
Cochlea;
metabolism;
Guinea Pigs;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Spiral Ganglion;
drug effects;
metabolism;
bcl-2-Associated X Protein;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2016;30(1):45-48
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effectiveness of cisplatin on the expressions of Bcl-2 and Bax in cochlea and spiral ganglion cells (SGC) of guinea pigs.
METHOD:Twenty guinea pigs were randomly divided into cisplatin (n = 10) and control groups (n = 10). Cisplatin group were administrated with a dose of intraperitoneal injection of 16 mg/kg, while the control group were received intraperitoneal injection of normal saline as placebo. Before and 7 days following injections, the ototoxic effect was measured with distortion product otoacoustic emission (DPOAE). Bcl-2, Bax in cochlea were detected by Western Blot. Immunohistochemical staining was used to detect the protein levels of Bcl-2 and Bax in spiral ganglion cells.
RESULT:In control and cisplatin group, Bcl-2 protein levels were 0.727 8 ± 0.016 9 and 0.467 6 ± 0.020 1, Bax protein levels were 0.384 8 ± 0. 0217 and 0.735 6 ± 0.022 3 in cochlea respectively, both P < 0.01. In Control and cisplatin group, the grey values of Bcl-2 in SGC were 99.00 ± 2.42 and 149.80 ± 2.37 respectively, the grey values of Bax were 154.50 ± 2.80 and 104.50 ± 3.09 respectively, both P < 0.05.
CONCLUSION:Decreased expression of Bcl-2 and increased expression of Bax may be involved in cisplatin-induced apoptosis in cochlea and SGC of guinea pigs.
