Relationship between Testin expression and clinicopathological characteristics in nasopharyngeal carcinoma patients.
- Author:
Shujun LI
;
Mingxue XUE
;
Lurong HAN
- Publication Type:Journal Article
- MeSH:
Carcinoma;
Cytoskeletal Proteins;
metabolism;
Genes, Tumor Suppressor;
Humans;
Immunohistochemistry;
LIM Domain Proteins;
metabolism;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
metabolism;
pathology;
RNA, Messenger;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2016;30(4):310-313
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of Testin gene, and analyze its possible relationship with the clinicopathological features of human nasopharyngeal carcinoma.
METHOD:The expression of Testin in nasopharyngeal carcinoma tissues were detected by immunohistochemistry methods, semi-quantitative reverse transcription polymerase chain reaction and Western blot. The correlations of Testin to clinicopathologic features of nasopharyngeal carcinoma were analyzed.
RESULT:The positive expression rate of Testin in NPC biopsy tissue was 37.8% (17/ 45), while it was 88.9% (40/45) in the normal tissue; The expression of Testin mRNA was significantly lower than that in the normal tissue (P < 0.01); The expression levels of Testin protein in the NPC biopsy tissue by Western blot were lower than that in the normal tissue (P < 0.01); The expression of Testin in the tumor tissue had no significant correlation with sex, age (P > 0.05); but it had significant correlation with lympho node metastasis, distant metastasis and differentiation degree.
CONCLUSION:The decreased expression of Testin gene may play an importmant role in the development of nasopharyngeal carcinoma. And thus Testin gene might be a novel candidate of tumor-suppressor. It may be an objective marker for prognostic factor and malignant level for nasopharyngeal carcinoma.
