Staphylococcus aureus biofilm influences the expression of lysozyme, SLPI and gp340 in a human sinonasal explant model.
- Author:
Ruiqing DI
;
Dong DONG
;
Lin YE
;
Yulin ZHAO
- Publication Type:Journal Article
- MeSH:
Biofilms;
Enzyme-Linked Immunosorbent Assay;
Ethmoid Sinus;
metabolism;
microbiology;
Humans;
Immunity, Innate;
Muramidase;
metabolism;
RNA, Messenger;
metabolism;
Receptors, Cell Surface;
metabolism;
Secretory Leukocyte Peptidase Inhibitor;
metabolism;
Staphylococcal Infections;
metabolism;
Tissue Culture Techniques
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2016;30(3):194-199
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the influences of staphylococcus aureus in planktonic and biofilm forms on the expression of lysozyme, SLPI and gp340 in the human sinonasal explant model.
METHOD:Mucosa samples from ethmoid sinus were collected from ten patients of cerebrospinal fluid leak and were cultured with and without S. aureus biofilms and planktonic cells. After the infection, the explant model was confirmed by CLSM, and the secretion of lysozyme, SLPI and gp340 was detected by enzyme-linked immunosorbent assay (ELISA) at 8, 16, and 24 h after S. aureus challenge. Expressions of lysozyme, SLPI and gp340 in mRNA and protein levels after 24 h S. aureus challenge were detected using RT-PCR, immunohistochemistry and Western bolt assay respectively.
RESULT:The secretion of lysozyme, SLPI and gp340 in the explant model was observed with a trend to increase in a time-dependent manner. At 8 and 16 h after S. aureus challenge, the secretion of lysozyme, SLPI and gp340 in biofilms group was significantly higher than these in planktonic cells group and control group (P<0. 05). S. aureus biofilms enhanced the mRNA expressions of lysozyme, SLPI and gp340 significantly compared with planktonic cells and controls, and the mRNA expressions in the explant model challenged by planktonic cells were significantly higher than controls (P < 0.05). Although the Western bolt assay showed no differences between the lysozyme expression in the planktonic cells group and control group (P > 0.05), the biofilms enhanced the expressions of lysozyme, SLPI and gp340 significantly compared with planktonic cells and controls (P < 0.05).
CONCLUSION:S. aureus biofilm induced the expressions of lysozyme, SLPI and gp340 to a higher level than planktonic cells, indicating that S. aureus biofilm was an influencing factor on the innate immune system.
