CD44 regulates epithelial-mesenchymal transition and metastasis in nasopharyngeal cancer cells.
- Author:
Shuang WANG
1
;
Shisheng LI
;
Dinghua XIE
;
Qinglai TANG
;
Shuhui WANG
;
Jiajia LIU
;
Yuehong CHEN
;
Xinming YANG
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, the Second Xiangya Hospital, Central South University, Changsha, 410011, China.
- Publication Type:Journal Article
- MeSH:
Carcinoma;
Cell Line, Tumor;
Epithelial-Mesenchymal Transition;
Humans;
Hyaluronan Receptors;
genetics;
metabolism;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
metabolism;
pathology;
Neoplasm Metastasis;
Transfection
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2013;27(5):250-254
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the correlation of CD44 with epithelial-mesenchymal transition(EMT) and metastasis in nasopharyngeal cancer cells, and explore the possible mechanism of CD44 regulates EMT and metastasis in nasopharyngeal cancer cells.
METHOD:The CD44 and EMT-associated proteins in 5-8F and 6-10B nasopharyngeal cancer cell lines were assayed by Western blotting. The erasion trace test was performed to observe the migratory ability of 5-8F and 6-10B nasopharyngeal cancer cells. Using lipid-mediated DNA transfection technique, the low metastatic nasopharyngeal cancer cells 6-10B were transfected in vitro with plasmid which contained CD44 gene, and then new nasopharyngeal cancer cells were obtained. The CD44 and EMT-associated proteins in 6-10B, empty vector transfected and CD44-transfected cells were assayed by Western blotting. The erasion trace test was performed to observe the alteration of migratory ability of nasopharyngeal cancer cells before and after CD44 transfection.
RESULT:The expression of CD44 and EMT-associated protein MMP-9 in 5-8F was higher than that in 6-10B, but EMT-associated protein E-Cadherin in 5-8F was lower than that in 6-10B. The migratory ability of 5-8F was higher than that of 6-10B. The expression of CD44 and MMP-9 were significantly higher in the CD44-transfected nasopharyngeal cancer cells than in the control groups. Compared with control groups, the migratory ability of CD44-transfected nasopharyngeal cancer cells was significantly increased.
CONCLUSION:CD44 positively regulates the metastatic ability of nasopharyngeal cancer cells, which is relevant to the process of EMT.
