Expression of transcription factors T-bet and GATA-3 and relationship to the numbers of eosinophils in rat model of allergic rhinitis.
- Author:
Chunping YANG
1
;
Chunlin LI
;
Gui LUO
;
Ying LUO
;
Yuehui LIU
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, the Second Affiliated Hospital, Nanchang University, Nanchang, 330006, China. artent@sina.com.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Count;
Eosinophils;
cytology;
Female;
GATA3 Transcription Factor;
metabolism;
Hypersensitivity;
immunology;
metabolism;
Male;
Rats;
Rats, Sprague-Dawley;
Rhinitis;
immunology;
metabolism;
T-Box Domain Proteins;
metabolism;
Th1 Cells;
metabolism;
Th2 Cells;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2011;25(23):1090-1093
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the expression of T-bet/GATA-3 in nasal mucosa tissue of allergic rhinitis rat and to investigate the association between the expression of T-bet/GATA-3 and the eosinophil count.
METHOD:Twenty SD rats were randomly divided into a control group and an allergic rhinitis group. The allergic rhinitis rat model was induced with ovalbumin. The total eosinophils were counted in the nasal mucosa. The concentrations of IL-4, IL-5 and IFN-gamma in nasal lavage fluid were measured by ELISA. The mRNA and protein expressions of IL-4, IL-5, IFN-gamma, T-bet and GATA-3 in the nasal mucosa were detected by RT-PCR and Western blot respectively.
RESULT:The main inflammatory cells were eosinophils in the nasal mucosa of allergic rhinitis rats. The level of IL-4, IL-5 and IFN-gamma in control group was significantly higher than that in allergic rhinitis group (P < 0.01). The mRNA and protein expression of IFN-gamma and T-bet in allergic rhinitis group was significantly higher than that in control group (P < 0.01). While the mRNA and protein expression of IL-4, IL-5 and GATA-3 in control group was significantly higher than that in allergic rhinitis group (P < 0.01). The ratio of protein expression of T-bet and GATA-3 was negatively correlated with the eosinophil count, IL-4 and IL-5, but positively with the concentrations of IFN-gamma.
CONCLUSION:The imbalance of transcription factor GATA-3 and T-bet has a close correlation with the eosinophil count, and may play a key role in the formation of allergic rhinitis.
