Micromorphological observation of bacterial biofilms on ciliated epithelia of chronic rhinosinusitis.
10.13201/j.issn.1001-1781.2012.05.005
- Author:
Haibin YANG
1
;
Yongqing GUO
;
Mingqiang YU
Author Information
1. Department of Otorhinolaryngology, Zhongshan Hospital, Xiamen University, Xiamen, 361004, China. iamyhb@hotmail.com
- Publication Type:Journal Article
- MeSH:
Bacterial Infections;
microbiology;
Biofilms;
Cilia;
microbiology;
ultrastructure;
Epithelium;
microbiology;
ultrastructure;
Humans;
Microscopy, Electron, Scanning;
Sinusitis;
microbiology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2012;26(5):194-196
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the micromorphological characteristic of bacterial biofilm on mucosa of chronic rhinosinusitis (CRS).
METHOD:Mucosa samples of middle turbinate were obtained from 4 patients of CRS during ESS. The size of each sample was about 4 mm x 4 mm. The samples were fixed in 4% paraformaldehyde for 24 hours, then fixed in 1% osmium tetroxide for 2 hours, graded dehydration with ethanol, dried with carbon dioxide and sputter coated with gold. The ultrastructure of these samples was observed by scanning electron microscope.
RESULT:Bacterial biofilms were found on samples in all 4 patients. The biofilms were mainly formed on the surface of cilia. The bacterial flagella and cilia were intertwined. The biofilms could be found in a lot kinds of bacterial infections or mixed infections which were caused by multiple bacteria and fungi.
CONCLUSION:Bacterial biofilm could be formed on ciliated epithelia.
