Skin prick testing versus immumofluorescence testing for mites allergens.
10.13201/j.issn.1001-1781.2012.05.010
- Author:
Yong ZHANG
1
;
Zhiyi WANG
;
Wei CHEN
;
Junfeng JI
;
Tianyou WANG
;
Qiuping WANG
;
Zeqing LI
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, Nanjing General Hospital of Nanjing Military Command, Nanjing 210002, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Animals;
Child;
Child, Preschool;
Female;
Fluorescent Antibody Technique;
methods;
Humans;
Immunoglobulin E;
blood;
Male;
Middle Aged;
Pyroglyphidae;
immunology;
Rhinitis, Allergic, Perennial;
diagnosis;
immunology;
parasitology;
Sensitivity and Specificity;
Skin Tests;
methods;
Young Adult
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2012;26(5):212-214
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To discuss the characteristics of skin prick test (SPT) and immumofluorescence method (IFM) in detecting the atopy of dermatophagoides pteronyssinus (Dp) and dermatophagoides farinae (Df)and explore the relationship of them.
METHOD:Four hundred and ninety-one patients with two or more symptoms of nasal obstruction,itching, sneezing and rhinorrhea received SPT and serum specific IgE detection using IFM.
RESULT:SPT was more sensitive than IFM in detecting the atopy of Dp and Df (P < 0.05). If using the IFM as the gold standard, the sensitivity of SPT was all above 95% with the specificity a little bit lower than 80%. These two methods had a positive correlation in detecting the atopy of Dp and Df (r = 0.779, 0.776; P < 0.01). The sIgE concentrations of these two mites were highly correlated with each other (r = 0.954, P < 0.01), the SPT was highly correlated with each other (r = 0. 946, P < 0.01).
CONCLUSION:SPT was more sensitive than IFM in detecting the atopy of Dp and Df. In most cases, SPT can replace IFM to detect patients' allergia on mites. Besides, it is good enough to detect either of the serum specific IgE concentrations of Dp and Df.
