Expression of Th1/th2 transcription factors and cytokines in laryngeal squamous cell carcinoma and its clinical significance.
- Author:
Gang DENG
1
;
Chengzhang YANG
;
Wangyan CHEN
Author Information
1. Department of Otorhinolaryngology,Chinese and Western Medicine Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. dgang2000@163.com
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Carcinoma, Squamous Cell;
metabolism;
pathology;
Case-Control Studies;
Female;
GATA3 Transcription Factor;
metabolism;
Humans;
Interferon-gamma;
metabolism;
Interleukin-4;
metabolism;
Laryngeal Neoplasms;
metabolism;
pathology;
Leukocytes, Mononuclear;
metabolism;
Male;
Middle Aged;
RNA, Messenger;
genetics;
T-Box Domain Proteins;
metabolism;
Th1 Cells;
metabolism;
Th2 Cells;
metabolism;
Tumor Suppressor Protein p53;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2012;26(6):270-273
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the differentiation of Th1/Th2 on the levels of transcription factors and cytokines production in patients with laryngeal squamous cell carcinoma in peripheral blood mononuclear cells (PBMC). In addition, the relation of p53 and T-bet, GATA3 expression was discussed for understanding the role of T-bet and GATA3 in metastasis.
METHOD:The gene expression of Th1/Th2 type transcription factors T-bet, GATA3 and cytokines IFN-gamma, IL-4 were determined by RT-PCR and realtime RT-PCR from 49 patients with laryngeal squamous cell carcinoma and 30 normal controls. The expression of p53 was analyzed by immunohistochemistry.
RESULT:Predominant expression of Th2 type cytokines was detected in 49 laryngeal squamous cell carcinoma patients. The expression rates of T-bet, GATA3, IFN-gamma and IL-4 was 42.86% (21/49), 71.43% (35/49), 26.53% (13/49), 63.27% (33/49) respectively. The expression rates of T-bet and IFN-gamma in patients were lower than in control group (P < 0.05), but the results of GATA3 and IL-4 were opposite (P < 0.05). The similar results were obtained by realtime PCR. The expression of p53 in patients was accompanied with lower expression of T-betmRNA and higher expression of GATA3mRNA.
CONCLUSION:There is predominant expression of Th2 type transcription factors and cytokines in PBMC of laryngeal squamous cell carcinoma patients. T-bet and GATA3 can be used as reference indicators for the metastasis of laryngeal squamous cell carcinoma.
