Mitochondrial mechanisms of antisense oligodeoxynucleotide Stat3 induced apoptosis in laryngeal carcinoma cell.
- Author:
Haili LÜ
1
;
Quihang ZHANG
;
Bo YAN
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, Xuanwu Hospital, Capital University of Medical Science, Beijing 100053, China. lvhaili7823@gmail.com
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Proliferation;
Cytochromes c;
metabolism;
Hep G2 Cells;
Humans;
Laryngeal Neoplasms;
genetics;
metabolism;
pathology;
Membrane Potential, Mitochondrial;
Mitochondria;
metabolism;
Oligodeoxyribonucleotides, Antisense;
genetics;
STAT3 Transcription Factor;
genetics;
Transfection;
Voltage-Dependent Anion Channels;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2012;26(7):316-318
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the changes of mitochondrion by transferring antisense oligodeoxynucleotide Stat3 into laryngeal carcinoma Hep-2 cell, for elucidating the mechanism of laryngeal carcinoma Hep-2 cell apoptosis, for developing more effective treatment for laryngeal cancer.
METHOD:The designed Stat3 ASODN was transferred into laryngeal carcinoma Hep-2 cell by lipofection. Mitochondrion membrane potential, VDAC and Cyt-c were detected for determining the changes of mitochondrion.
RESULT:MMP was fell, Cyt-c and VDAC were increased with the heighten concentration of ASODN.
CONCLUSION:Mitochondria approach play an important role in the apoptosis mechanism of human Hep-2 cell by Stat3. This research elucidated the regulating mechanism of Hep-2 cell proliferation by Stat3, provided a new research focus for clinical therapy.
