Synergistic interactions of TRAIL and paclitaxel on the nasopharyngeal carcinoma cell lines in vitro.
- Author:
Pingqing TAN
1
;
Yong LIU
;
Yuanzheng QIU
;
Shisheng LI
;
Ying GUO
;
Gao LI
;
Yongquan TIAN
;
Xin ZHANG
;
Donghai HUANG
;
Jianyun XIAO
Author Information
1. Department of Otorhinolaryngology, Xiangya Hospital, Central South University, Changsha 410008, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Carcinoma;
Cell Line, Tumor;
Humans;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
pathology;
Paclitaxel;
pharmacology;
Receptors, TNF-Related Apoptosis-Inducing Ligand;
pharmacology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2012;26(7):319-322
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the synergistic cytotoxicity of TRAIL and paclitaxel on nasopharyngeal cell lines CNE-1 and CNE-2.
METHOD:CCK-8 assays the growth inhibition rate of CNE-1 and CNE-2 which was treated with TRAIL or paclitaxel or combination of both. Flow cytometry tests the apoptosis rate of CNE-1 and CNE-2 which was treated with TRAIL or paclitaxel or combination of each other.
RESULT:In certain range of time and concentration,TRAIL and paclitaxel inhibited the growth of the cell lines of CNE-1 and CNE-2 in a time-dose dependent manner (P < 0.05). The rate of growth inhibition and apoptosis in TRAIL and paclitaxel combinative group was more significant than that in the TRAIL and paclitaxel singular group (P < 0.05).
CONCLUSION:TRAIL and paclitaxel had a synergistic killing effect on NPC cell lines and showed better affection than singular group, which provides a novel and prospective strategy for NPC chemotherapy.
